Identification of Unknown Carbohydrates | Lab Report

realization of Unknown Carbohydrates Lab rootINTRODUCTIONOne of the main types of nutrients is the carbohydrates. Carbohydrates are the most vital psychiatric hospital of force for your body. Our digestive system has a capacity to change carbohydrates into glucose or most commonly known as blood sugar. Our body gets energy used by our cells, tissues and organs from this sugar. Carbohydrates also stores additional sugar in our liver and muscles.Carbohydrates may be unbiased or mixed depending on its chemical structure. Simple carbohydrates are also known as simple sugars. They are commonly established in refined sugar such as white sugar. Complex carbohydrates of amylumes includes grain products like bread, crackers, pasta and rice.MATERIALS AND METHODSA. Identification of Unknown Carbohydrate SamplesApproximately 1.00 ml of the known carbohydrate stresss and the devil unknown models were transferred on separate labelled trial tubes. About 1.00 ml of Molisch reagent then 1.00 ml of concentrated H2SO4 was added to each sample. The test was observed for any(prenominal) change and was recorded. With the use of new batch of samples each time, the remaining tests were conducted(a) ace test 1.00 ml of iodine reagent was added to each sample.(b) benedicks test 1.00 ml of Benedict reagent was added to each sample then alter use weewee bath.(c) Barfoeds test 1.00 ml of Barfoeds reagent was added to each sample then heated using water bath.(d) Seliwanoffs test 1.00 ml of Seliwanoff reagent was added to each sample then heated using water bath.(e) 2,4-DNP test 1.00 ml of 2,4-DNP was added to each sample then heated using water bath.The identity of the unknown samples was determined by comparing it to the known carbohydrate samples.B. Hydrolysis of StarchExactly 50.0 ml of 5% starch solution was transferred in a 100-ml beaker. Precisely 5.00 ml of concentrated sulfuric acid or hydrochloric acid was added. The sample was covered with aluminium hy bridise and was heated using water bath. Two 1.00 ml volume of the sample were transferred in a test tube. Exactly 1.00 ml of iodine reagent was added to integrity tube and 1.00 ml of Benedicts reagent was added to the other. The reaction was observed. The sample was heated continuously. Two 1.00 ml volume of the sample was transferred between every 5 minute legal separation and tested with iodine and Benedicts reagent as above until bodyation of blue-black complex in iodine stops and formation of brick red colour in Benedicts reagent ensues.RESULTS AND DISCUSSIONIn Molisch test, the result turned out to be confirming or slow reaction. It is because of the formation of the reaction with alpha-naphthol in the occurrence of sulfuric acid. In this test, every type of carbohydrates will crap a collateral result.Benedicts solution is a deep-blue alkaline solution used in interrogation the existence of the aldehyde functional group, -CHO. Benedicts reagent consists of blue blur ( II) ions which are condensed to copper (I).These ions will then be precipitated as red copper (I) oxide which is not soluble in water. In Benedicts test, monosaccharides and disaccharides except for sucrose give a positive result. It is when the result is a brick red precipitate.In Barfoeds test, the copper ion in solution oxidizes reducing monosaccharides. This is for the formation of a carboxylic acid and red precipitate of copper (I) oxide in 3 minutes.In Seliwanoffs test, the reagent dehydrates ketohexoses to form 5-hydroxymethylfurfural which will further react with resorcinol, that is present in the reagent, to produce a red product in 2 minutes.In Iodine test, all polysaccharides such as glycogen and starch give positive result. The sample turns to blue-black color.